Scientific Papers and Reports

This is an annotated list of the published papers and reports that have included REEF data. The list is in chronological order. Papers that are available for viewing in .pdf format are noted.

Also see the Projects page for links to additional reports.

Green SJ, JL Akins, and IM Côté. 2011. Foraging behaviour and prey consumption in the Indo-Pacific lionfish on Bahamian coral reefs.

Marine Ecology Progress Series. 433:150-167

Predicting and mitigating the effects of invasive Indo-Pacific lionfish Pterois volitans on Caribbean fish communities requires a thorough understanding of the species’ predation behaviour in the invaded range, including the types and amounts of prey consumed and how foraging patterns vary in relation to extrinsic conditions. We studied the activity levels and prey consumption rates of lionfish on 12 shallow coral reefs in the Bahamas in relation to time of day and prey availability. Lionfish predation rates and activity levels were significantly higher during crepuscular (dawn and dusk) periods than at mid-day. Available prey fish biomass was highest at dawn but lower at mid-day and dusk, suggesting that lionfish predation activity is not limited by prey availability alone. Our calculated average daily mass-specific prey consumption rates, which incorporated daily variation, was ~3 times the estimates obtained from studies of captive lionfish in their native range and of invasive lionfish observed only during the day. Our results will help to predict more accurately the effect of predation by invasive lionfish on native reef fish communities.

Auster, P.J., D. Grenda, J. Godfrey, E. Heupel, S. Auscavitch, and J. Mangiafico. 2011. Behavioral Observations of Lilliputian Piscivores: Young-of-Year Sphyraena barracuda at Offshore Sub-Tropical Reefs (NW Atlantic.

Southeastern Naturalist. 10(3):563-569

REEF Advanced Assessment Team Member, Dave Grenda, recently co-authored a paper documenting behavioral observations of young Great Barracuda occurring on live bottom sub-tropical reefs primarily at Gray's Reef National Marine Sanctuary off the coast of Georgia. For the past three years Dave assisted University of Connecticut researcher, Dr. Peter Auster, in studying behavioral interactions of piscivores and their prey. During REEF surveys on these cruises, Dave and the other researchers documented young-of-year (YOY) Great Barracuda (those individuals that had very recently settled to the reef, and were between 2-3 inches in length) hunting YOY Tomtate and Silverside that were taking refuge under ledges. Groups of YOY Barracuda would attack, capture, and consume the prey. Prey that escaped the Barracuda retreated to reef edges and were often consumed by bottom-dwelling adult piscivores such as Black Sea Bass, Bank Sea Bass, and Scamp Grouper. These findings indicate that given the strong functional role the young Barracuda have on driving species interactions, greater attention should be given to the roles played by the wider diversity of YOY piscivores recruiting to reef communities.

Koenig, CC, FC Coleman, and K Kingon. 2011. Pattern of Recovery of the Goliath Grouper (Epinephelus itajara) Population in the Southeastern US.

Bulletin of Marine Science. 87: doi:10.5343/bms.2010.1056

This paper reviewed the population status of Atlantic goliath grouper, Epinephelus itajara, in coastal waters of the southeastern United States. The study is based on quantitative surveys conducted by the authors and the Florida Fish and Wildlife Conservation Commission’s Artificial Reef Program (n = 505), coupled with data submitted by REEF surveyors (n = 27,542) over 15+ yrs. The primary objective of the study is to describe the distribution and abundance of the goliath grouper population of the southeastern US with a focus on Florida, the center of US abundance for this species, the center for the historical fishery, and now the center of much controversy. A secondary, but quite important objective is to demonstrate the utility of using existing databases, such as that of REEF to provide what often amounts to the best scientific data available for informing management decisions, particularly in areas and for species for which there is no traditional fishery- dependent data collection.

The authors found that the population of goliath grouper, after dramatic fishery-induced declines in the 1970s and 1980s, and eventual fishery closure in the 1990s, increased off southwest Florida in the mid-1990s, directly offshore of the high-quality mangrove nursery of the Ten Thousand Islands. It then expanded north and south, eventually increasing off Florida’s central east coast. The study also evaluated tagging data, which showed that, regardless of life stage, individuals showed strong site fidelity to home sites: juveniles to mangrove nursery sites and adults to offshore reefs. All long-distance movements appeared to be in response to approaching maturity, with juveniles emigrating from mangroves to take up residence on offshore reefs, to seasonal spawning activity, with adults moving from home sites to aggregation sites, or to apparent feeding sites in inlets. Understanding these patterns of population recovery and movement is fundamental to devising appropriate management policies.

Holt, B., I Cote, and B Emerson. 2010. Signature of speciation? Distribution and diversity of Hypoplectrus (Teleostei: Serranidae) colour morphotypes..

Global Ecology and Biogeography. 19: 432–441

Hamlets are a group of colourful coral reef fish found throughout the Caribbean. Ten species of hamlet have been discovered and each can be easily recognized by its own distinct colour pattern. In some areas, as many as seven varieties can be found on a single reef. However, most hamlet species are only found at specific locations. The blue hamlet, for example, is found only in the Florida region. How these very different looking, yet very closely related species came to be has been a a subject of debate among scientists. Data collected by divers and snorkelers as part of the REEF Volunteer Fish Survey Project were used in a large analysis to better understand the patterns of evolution in these and other marine fishes. They found that even widespread hamlet species are not found everywhere, and identified high density hotspots for each species. Because different species hotspots overlap and many species have more than one hotspot, the results do not support the theory that hamlets originated independently when they were geographically separated in the past. The research also showed how ecological factors, such as competition for food or habitat, may influence how different hamlet species co-exist. To contact the lead author -

Victor, B.C.. 2010. The Redcheek Paradox: the mismatch between genetic and phenotypic divergence.

Journal of the Ocean Science Foundation. Vol 3, 15 April 2010

Long-time REEF supporters, Les and Keri Wilk of ReefNet, recently discovered and photographed a distinctively marked population of the Greenbanded Goby, Elacatinus multifasciatus, on the island of Utila, Honduras. The population was distinguished by a prominent red stripe across the cheek that is not found on other populations of Greenbanded Gobies, as well as more numerous green bars on the body. The Wilks contacted Dr. Ben Victor, a reef fish taxonomic expert, who conducted a regional genetic comparison of Greenbanded Gobies to evaluate hidden diversity within this colorful and cryptic reef fish. As part of the study, the REEF database was used to document the current geographic range of the species. Ben's results identified the unique looking fish to be a separate species that is now called the Redcheek Goby (E. rubrigenis). He also discovered that, based on genetic results, Greenbanded Goby along coastal Panama, despite looking just like others in the species, are most likely a distinct species that will now be called Panamanian Greenbanded Goby (E. panamensis)

Ward-Paige CA, Mora C, Lotze HK, Pattengill-Semmens C, McClenachan L, et al.. 2010. Large-Scale Absence of Sharks on Reefs in the Greater-Caribbean: A Footprint of Human Pressures.

PLoS ONE. 5(8): e11968. doi:10.1371/journal.pone.0011968

In recent decades, large pelagic and coastal shark populations have declined dramatically with increased fishing; however, the status of sharks in other systems such as coral reefs remains largely unassessed despite a long history of exploitation. The authors used REEF data to explore the contemporary distribution and sighting frequency of sharks on reefs in the greater-Caribbean, and assessed the possible role of human pressures on observed patterns. The analysis was based on 76,340 underwater surveys carried out by REEF volunteers between 1993 and 2008. The authors compared sighting frequency to the number of people in each area surveyed, and used population viability analyses to assess the effects of exploitation on population trends. Sharks, with the exception of nurse sharks occurred mainly in areas with very low human population or strong fishing regulations and marine conservation. Population viability analysis suggests that exploitation alone could explain the large-scale absence; however, this pattern is likely to be exacerbated by additional anthropogenic stressors, such as pollution and habitat degradation, that also correlate with human population. Preventing further loss of sharks requires urgent management measures to curb fishing mortality and to mitigate other anthropogenic stressors to protect sites where sharks still exist. The fact that sharks still occur in some densely populated areas where strong fishing regulations are in place indicates the possibility of success and encourages the implementation of conservation measures.

Ward-Paige, C.A., C.V. Pattengill-Semmens, R.A. Myers, and H.K. Lotze. 2010. Spatial and temporal trends in yellow stingray abundance: evidence from diver surveys.

Environmental Biology of Fishes. 90:263–276

Recent concerns about changing elasmobranch populations have prompted the need to understand their patterns of distribution and abundance through non-destructive sampling methods. Since scientific divers represent a small portion of the total number of divers worldwide, the use of non-scientific divers could drastically increase the number of observations needed to monitor broad-scale, long-term trends. Here, we use 83,940 surveys collected by trained volunteer divers to examine spatial and temporal trends of the most frequently sighted elasmobranch species in the greater-Caribbean, the yellow stingray (Urobatis jamaicensis). Despite being relatively common and listed as Least Concern on the IUCN Red List, little is known about the status of this species. In total, yellow stingrays were observed on 5,658 surveys (6.7% sighting frequency) with the highest occurrence in the regions surrounding Cuba. Overall, sighting frequency declined from 20.5% in 1994 to 4.7% in 2007—a standardized decline rate of −0.11. However, these trends were not consistent in all regions. The strongest decline occurred in the Florida Keys, the most sampled region, where trends were similar among all areas, habitats and depths. In contrast, sighting frequency significantly increased in Jamaica where large fishes are severely depleted. We discuss possible explanations for these changes including habitat degradation, exploitation and changes in trophic interactions. Our results suggest large-scale changes in yellow stingray abundance that have been unnoticed by the scientific community. Thus, our study highlights the value of non-scientific divers for collecting data that can be used to understand population trends of otherwise poorly studied species.

Freshwater, D.W., A. Hines, S. Parham, A. Wilbur, M. Sabaoun, J. Woodhead, L. Akins, B. Purdy, P.E. Whitfield, and C.B. Paris. 2009. Mitochondrial control region sequence analyses indicate dispersal from the US East Coast as source of invasive Indo-Pac Lionfish.

Marine Biology. 28 February 2009

This paper examined the genetic source of the invasive Indo-Pacific lionfish in the Bahamas. Many of the samples used in this study were collected during REEF's lionfish research trips during 2007 and 2008. Two species, Pterois volitans and P. miles, were well established along the United States east coast before the first lionfish were reported from the Bahamas in 2004, where they quickly dispersed throughout the archipelago by 2007. The source of the Bahamian lionfish invasion has been in question because of the hypothesized low connectivity between Florida and Bahamas reef species as well as the temporal lag in their arrival in the Bahamas. The results showed no significant differentiation between the Bahamas and North Carolina specimens. Sequence analyses also revealed the presence of only Pterois volitans in the Bahamas, with no P. miles detected in any of the specimens. These results indicate that the source of the Bahamian lionfish is egg and larval dispersal from the United States east coast population, and support previous models of reef fish dispersal that suggest a low level of connectivity between the Bahamas and east coast of Florida.

Stallings, C. 2009. Fishery-Independent Data Reveal Negative Effect of Human Population Density on Caribbean Predatory Fish Communities.

PLoS One. 4(5): e5333

Understanding the current status of predatory fish communities, and the effects fishing has on them, is vitally important information for management. However, data are often insufficient at region-wide scales to assess the effects of extraction in coral reef ecosystems of developing nations. The author overcomes this difficulty by using REEF's large database from the Volunteer Survey Project, which is a publicly accessible, fisheries-independent database. The aim of the study was to provide a broad scale, comprehensive analysis of human impacts on predatory reef fish communities across the greater Caribbean region. Specifically, this study analyzed presence and diversity of predatory reef fishes over a gradient of human population density. Across the region, as human population density increases, presence of large-bodied fishes declines, and fish communities become dominated by a few smaller-bodied species. The analysis found a complete disappearance of several large-bodied fishes, which indicates ecological and local extinctions have occurred in some densely populated areas. These findings fill a fundamentally important gap in our knowledge of the ecosystem effects of artisanal fisheries in developing nations, and provide support for multiple approaches to data collection where they are commonly unavailable.

Biological Review Team NWFSC. 2009. Preliminary Scientific Conclusions of the Review of the Status of 5 Species of Rockfish.

This was a predecision document prepared by NOAA’s Fisheries Service to evaluate the population status of five species of rockfish in the Puget Sound. REEF data were one of several datasets that were used by the fisheries scientists to make the evaluation. Based on the analyses, NOAA Fisheries proposed to list three populations of rockfish in Puget Sound and the Strait of Georgia for protection under the Endangered Species Act. The Georgia Basin populations of two of the rockfish species – canary and yelloweye – are proposed for “threatened” status. A third rockfish species – bocaccio – is proposed as “endangered.” Populations of all three rockfish species in the Georgia Basin, which encompasses Puget Sound and the Strait of Georgia, have been harvested at high levels, depleting their numbers. Rockfish, which are bottom dwellers, typically live long lives, and mature and reproduce slowly, making them especially vulnerable to overfishing. A final decision on the three will be made in the Spring 2010.

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